Thermo Fisher Scientific CD95/FAS/TNFRSF6 Monoclonal Antibody (FAS, 3112)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein functioning as a cell death receptor in the TNFR (tumor necrosis factor receptor) superfamily. A soluble form (~26 kDa) also exists. FAS activation leads to aggregation of FADD domains, formation of the death-inducing signaling complex (DISC), and caspase activation, resulting in apoptosis.
FAS signaling can activate NF-κB, MAPK3/ERK1, and MAPK8/JNK pathways, and is involved in proliferation signaling in fibroblasts and T cells. Multiple alternatively spliced variants exist, some lacking the transmembrane domain, which may regulate apoptosis negatively.
The Fas/Fas ligand system is implicated in diseases such as AIDS, hepatitis, and cancer, and plays a key role in antiviral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.