Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (ACK2), eBioscience
Mouse CD117 (c-Kit) 인식 단일클론 항체로, hematopoietic progenitor cell 연구에 적합. Flow cytometry, WB, IHC 등 다양한 응용 가능. 비결합형 Rat IgG2b 항체로 0.5 mg/mL 농도, PBS buffer에 sodium azide 포함. Blocking 및 functional assay용으로 사용 가능.
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (ACK2), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 3 publications |
| Immunohistochemistry (IHC) | - | 26 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 12 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | 35 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 1 publication |
| Functional Assay | Assay-Dependent | 2 publications |
| Inhibition Assay (IA) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Chicken, Fish, Guinea pig, Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ACK2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467435 |
Additional Formats Available
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 702
- Custom conjugation available upon request
Product Specific Information
Description:
ACK2 monoclonal antibody reacts with mouse CD117 (c-Kit receptor, Steel factor receptor, stem cell factor receptor). This 145 kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells and mast cells, playing a key role in proliferation, differentiation, and activation. ACK2 is reported to be a blocking antibody.
Applications Reported:
Suitable for flow cytometry, immunoprecipitation, Western blot, and immunohistochemistry of frozen tissue sections. Also used in functional assays (Functional Grade purified ACK2 recommended for functional assays).
Applications Tested:
Validated by flow cytometric analysis of mouse bone marrow cells. Recommended usage ≤0.125 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Titration recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (stem cell factor). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Activation of c-Kit by SCF induces receptor dimerization and autophosphorylation, triggering downstream signaling pathways (Ras/ERK, PI3K, PLC-γ, JAK/STAT). KIT is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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