Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (YB5.B8), eBioscience
CD117 (c-Kit) 단백질을 인식하는 YB5.B8 단클론 항체로, 인간 시료에 반응합니다. Flow Cytometry, Immunohistochemistry, Immunoprecipitation 등에 사용 가능하며, 0.5 mg/mL 농도의 액상 형태입니다. PBS buffer에 sodium azide 포함, 4°C 보관.
- 카탈로그번호
- 14-1179-xx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (YB5.B8), eBioscience
Applications
Immunohistochemistry (Frozen) (IHC (F))
- Assay-Dependent
Flow Cytometry (Flow)
- 1 µg/test
- Tested on TF-1 cell line (human erythroleukemia)
- Recommended titration for optimal performance
Immunoprecipitation (IP)
- Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | YB5.B8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864978 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
The YB5.B8 monoclonal antibody reacts with human CD117 (c-Kit), also known as Steel factor receptor or stem cell factor receptor. This 145 kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell development. CD117 is also expressed by melanocytes, playing a role in signaling and activation.
Applications Reported: Flow cytometry, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.
Target Information
KIT (c-KIT) is a proto-oncogene and a type III transmembrane receptor for mast cell growth factor (MGF, stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. Together with its ligand, KIT regulates growth and activation of various hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Deregulation of KIT receptor tyrosine kinase by mutation D816V is a key focus in therapeutic strategies for neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon binding of SCF, c-Kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues (e.g., Tyr703), enabling downstream signaling such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT pathways. c-Kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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