Thermo Fisher Scientific CD115 (c-fms) Monoclonal Antibody (AFS98), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563
• Functional Grade
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 610
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocytes, macrophages, osteoclasts, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to the immunoglobulin family. CSF-1 signaling through CSF-1R regulates proliferation and differentiation of cells in the monocytic lineage.

Applications Reported:
The AFS98 antibody has been reported for use in flow cytometry, Western blotting, and immunohistochemical staining of frozen tissue sections. It has also been reported in ligand binding blocking assays. For functional assays, use the Functional Grade purified AFS98 (Product #16-1152).

Applications Tested:
The AFS98 antibody has been tested by blocking of fluorochrome-conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. Recommended use ≤1 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.

Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family. Mutations in CSF1R are associated with a predisposition to myeloid malignancy. It acts as a receptor for CSF1 and IL34, regulating survival, proliferation, and differentiation of hematopoietic precursor cells, particularly mononuclear phagocytes. It also promotes proinflammatory chemokine release in response to IL34 and CSF1, playing an important role in innate immunity and inflammation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.