Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), eBioscience
Mouse CD117 (c-Kit) 단백질을 인식하는 Rat monoclonal antibody (Clone 2B8)로, Flow Cytometry 및 Immunoprecipitation에 적합합니다. Affinity chromatography로 정제되었으며, PBS buffer에 0.09% sodium azide를 포함하고 4°C에서 보관합니다. 다양한 형광 및 효소 결합 포맷으로 제공됩니다.
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- 14-1171-8x (3개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 10 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 3 |
| Immunocytochemistry (ICC/IF) | - | 6 |
| Flow Cytometry (Flow) | 0.5 µg/test | 121 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Pig |
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467432 |
Available Conjugated Formats
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737
- Brilliant Violet 421 / 480 / 605 / 650 / 786
- eFluor 450
- FITC
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed in hematopoietic progenitor cells, mast cells, and plays a critical role in their proliferation, differentiation, and activation.
- Applications Reported: Flow cytometric analysis and immunoprecipitation.
- Applications Tested: Verified for flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.5 µg/test (100 µL final volume).
- Purity: >90% (SDS-PAGE).
- Aggregation: <10% (HPLC).
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (MGF), also known as stem cell factor (SCF). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Upon SCF binding, c-Kit dimerizes and autophosphorylates, activating downstream signaling pathways such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT. This receptor is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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