Thermo Fisher Scientific HCN4 Monoclonal Antibody (N114/10), APC
HCN4 단백질을 검출하기 위한 APC 결합 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 사람, 생쥐, 토끼, 랫트 반응성. 단백질 G로 정제된 액상 형태로 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N114/10 |
| Immunogen | Fusion protein amino acids 1019–1198 (C-terminus) of rat HCN4 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931885 |
Additional Formats
- FITC (Cat. No. MA5-45432)
- PerCP (Cat. No. MA5-45433)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45431 was sufficient for detection of HCN4 in 10 µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 130 kDa. No cross-reactivity against other HCNs.
Target Information
HCN4 is a member of the hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channel family. HCN currents are linked to pacemaker activity in the heart and brain, resting potential control, and neuronal plasticity. HCN4 channels function as receptors for sour taste and are associated with pacemaker potential generation in the sinoatrial node.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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