Thermo Fisher Scientific TRPM7 Monoclonal Antibody (S74), FITC
TRPM7 단백질을 검출하기 위한 FITC 결합 단클론 항체. Western blot, IHC, ICC 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 단백질 G로 정제된 액상 형태로 제공되며, 4°C 암소 보관. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S74 |
| Immunogen | Fusion protein amino acids 1817–1863 (C-terminus) of mouse TrpM7 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931878 |
Additional Formats
- Unconjugated (MA5-27620)
- APC (MA5-45423)
- PE (MA5-45426)
- PerCP (MA5-45425)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45424 was sufficient for detection of TrpM7 in 10 µg of COS cell lysate transiently transfected with TrpM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 220 kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.
Target Information
TRPCs are mammalian homologs of the Drosophila transient receptor potential (trp) protein and function as ion channels mediating capacitative calcium entry. TRP-PLIK acts as both an ion channel and a kinase, conducting calcium and monovalent cations to depolarize cells and increase intracellular calcium. The kinase activity is essential for channel function, dependent on intracellular ATP and demonstrated by kinase mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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