Thermo Fisher Scientific HCN4 Monoclonal Antibody (N114/10), FITC
HCN4 단백질을 검출하기 위한 FITC 결합 마우스 단클론 항체입니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 인간, 마우스, 토끼, 랫트 반응성을 가지며, 고순도 Protein G 정제 및 형광 특성(498/517 nm)을 제공합니다.
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Thermo Fisher Scientific HCN4 Monoclonal Antibody (N114/10), FITC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N114/10 |
| Immunogen | Fusion protein amino acids 1019–1198 (C-terminus) of rat HCN4 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931886 |
Additional Formats
- APC (MA5-45431)
- PerCP (MA5-45433)
- Request custom conjugation
Product Specific Information
1 µg/mL of MA5-45432 was sufficient for detection of HCN4 in 10 µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 130 kDa. No cross-reactivity against other HCNs.
Target Information
HCN4 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents are linked to pacemaker activity in the heart and brain, resting potential control, and neuronal plasticity. HCN4 channels also function as receptors for sour taste and are associated with pacemaker potential generation in the sinoatrial node.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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