Thermo Fisher Scientific EPCAM Monoclonal Antibody (OTI8A9), TrueMAB
EpCAM 단백질을 인식하는 Mouse IgG1 단일클론 항체로 인간 시료에 반응합니다. Western blot에 최적화되어 있으며, 무보존제·동결건조 형태로 제공됩니다. 재구성 및 결합 실험에 적합하며, 세포 접착 및 종양 표지 연구용으로 사용됩니다.
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Applications
- Western Blot (WB)
Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8A9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 24–265 of human EPCAM produced in SF9 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7 K MWCO, 0.5 mL, Product #89882).
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It mediates Ca²⁺-independent homotypic cell adhesion and influences cell cycle, proliferation, and metabolism through induction of c-myc and cyclin A/E.
Ep-CAM overexpression correlates with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors. It is localized on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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