Thermo Fisher Scientific NES Monoclonal Antibody (OTI1B4), TrueMAB
인간 Nestin 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB 및 IHC(P)에서 검증됨. 재조합 단백질(aa 99–349)을 면역원으로 사용. Lyophilized 형태로 제공되며, PBS buffer에 8% trehalose 포함. 줄기세포 및 신경전구세포 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 99–349 of human NES produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system (CNS) and early embryonic neuroepithelial stem cells.
It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin is also recognized as a superior angiogenic marker for evaluating neovascularity in tumors.
Highly specific antibodies to human Nestin are useful for:
- Characterizing progenitor cells differentiating into distinct lineages
- Enhancing therapeutic potential of human neural stem/progenitor cells in CNS disease or injury
- Identifying neuroepithelial tumor cells
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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