Thermo Fisher Scientific NCF4 Monoclonal Antibody (OTI2D4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The NCF4 gene encodes a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system essential for host defense. It is preferentially expressed in myeloid lineage cells and interacts with NCF2 (p67-phox) and NCF1 (p47-phox) to form a complex that activates flavocytochrome b upon cell stimulation. The PX domain binds phospholipid products of PI(3) kinase, suggesting a role in PI(3) kinase-mediated signaling. Phosphorylation of this protein negatively regulates enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.