Takara MiraCell Endothelial Cells (from ChiPSC12) Kit

The MiraCell Endothelial Cells (from ChiPSC12) Kit contains highly pure (>95% CD31-positive) vascular endothelial cells derived from human induced pluripotent stem (iPS) cells, purified without using antibiotic or metabolic selection.

The MiraCell Endothelial Cells (from ChiPSC12) Kit contains highly pure (>95% CD31-positive) vascular endothelial cells derived from human induced pluripotent stem (iPS) cells, purified without using antibiotic or metabolic selection. In addition to expression of vascular endothelial markers such as CD31, Tie2, vWF (von Willebrand factor), and CD144 (VE cadherin), vascular endothelial cell functions such as Dil-Ac-LDL uptake activity and endothelial tube formation ability are stably maintained between different manufacturing lots. Therefore, these cells can be used for characterization and functional analysis of endothelial cells, toxicity testing in drug discovery, and regenerative medicine research. This kit contains one vial of endothelial cells (>1.5 x 106 viable cells) and growth medium components. The components for culturing the cells are also available separately as Cat. # Y50053.

This kit was jointly developed with iHeart Japan Corporation, and utilizes an endothelial cell production technique through which highly pure endothelial cells are prepared without antibiotic or metabolic selection, which was developed by the Center for iPS Cell Research and Application at Kyoto University.

The Cellartis human iPS cell line ChiPSC12 (Cat. # Y00285), cultured under feeder-free conditions using the Cellartis DEF-CS500 Culture System (Cat. # Y30010), was used in the manufacture of these cells.

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Overview

• High purity (>95% CD31-positive)
• Purified without antibiotic or metabolic selection
• Endothelial tube formation ability
• Dil-Ac-LDL uptake activity
• Expresses a wide variety of endothelial cell genes

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Applications

• Characterization and functional analysis of endothelial cells
• Toxicity testing in drug discovery
• Regenerative medicine research

Components

• MiraCell Endothelial Cells (from ChiPSC12) (>1.5 x 106 viable cells)
• MiraCell EC Culture Medium (500 ml)
• MiraCell EC Culture Supplement (6.5 ml x 2)

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.