Thermo Fisher Scientific Mouse IgM Isotype Control (11E10), PE-Cyanine7, eBioscience
Flow cytometry용 Mouse IgM 동형 대조 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. LPS로 면역화된 마우스에서 유래하였으며, 실험 항체와 동일 농도로 사용 권장됩니다. 광민감성이 있으므로 어둡게 보관하십시오.
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Applications
Tested Dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgM |
| Recommended Isotype Control | CD15 Monoclonal Antibody (HI98), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | 11E10 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811781 |
Additional Formats
Product Specific Information
The mouse IgM is useful as an isotype control immunoglobulin. It was generated by immunization with lipopolysaccharide (LPS) and is reactive with LPS.
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Tested on mouse splenocytes and human peripheral blood cells. Use at the same concentration as the experimental antibody.
- Light Sensitivity: PE-Cyanine7 is sensitive to photo-induced oxidation. Protect from light.
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark.
- Excitation / Emission / Laser: 488–561 nm / 775 nm / Blue, Green, Yellow-Green Lasers.
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody generated during an immune response and is the largest immunoglobulin in serum. Composed of five heavy and five light chains, it plays a critical role in complement activation and antigen agglutination. Mouse IgM is widely used in ELISA, Western blotting, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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