Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), PE-Cyanine7, eBioscience
인간 및 비인간 영장류 FOXP3 단백질을 검출하는 단클론 항체로, PE-Cyanine7 형광체가 결합되어 흐름세포분석에 최적화됨. CD4+CD25+ Treg 세포 분석에 사용되며, 488–561 nm 여기 및 775 nm 방출 특성을 가짐. 연구용으로만 사용.
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Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), PE-Cyanine7, eBioscience™
Applications and Tested Dilution
- Immunohistochemistry (Paraffin): Not specified
View 2 publications - Flow Cytometry: 5 µL (0.125 µg)/test
View 14 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rhesus monkey |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 236A/E7 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573449 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- eFluor 450
- eFluor 570
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- Custom conjugation available
Product Specific Information
The 236A/E7 antibody reacts with human Foxp3 protein (FORKHEAD BOX P3, SCURFIN, JM2). Foxp3 is a 49–55 kDa transcriptional regulator, identified as the gene defective in scurfy mice. High expression of Foxp3 is found in CD4+CD25+ regulatory T cells (Treg), and ectopic expression imparts a Treg phenotype.
Intracellular staining of human PBMCs with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523) reveals staining of the CD4+CD25bright population.
The epitope recognized by 236A/E7 differs from PCH101 (Product #72-5776). This antibody also recognizes rhesus macaque, sooty mangabey, and cynomolgus macaque.
- Applications Reported: Intracellular staining followed by flow cytometric analysis
- Applications Tested: Pre-titrated and validated for intracellular staining and flow cytometry using Foxp3/Transcription Factor Staining Buffer Set
- Recommended Use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples may be stored up to 3 days at 4°C using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
Excitation: 488–561 nm; Emission: 775 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is 381 amino acids long and constitutively expressed in CD4+CD25+ regulatory T cells. FOXP3 defects cause the scurfy phenotype in mice and contribute to human IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked). FOXP3 expression is regulated by p53 in response to DNA damage in certain cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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