Gibco™

RPMI 1640 Medium, GlutaMAX™ Supplement

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and자세히 알아보기

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:

With	Without
• GlutaMAX™	• HEPES
• Phenol Red

The complete formulation is available.

Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12, and PABA, which are not found in Eagles Minimal Essential Medium or Dulbeccos Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product intended use
For in vitro diagnostic use. CAUTION: not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
RPMI 1640 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (61870-010). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.

This product is not used for in vitro diagnostic purpose in some countries.

사양

세포주HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas

세포 유형Leukemic Cells

분류Animal Origin-free

농도1 X

형태Liquid

제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Serum LevelStandard Serum Supplementation

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함GlutaMAX, Phenol Red

첨가제 없음No HEPES, No Sodium Pyruvate

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™, GlutaMAX™

수량500 mL

배송 조건Room Temperature

Unit SizeEach