Thermo Fisher Scientific Alkaline Phosphatase (Placental)/PLAP (Germ Cell Tumor Marker) Monoclonal Antibody (PL8-F6)
인체 PLAP 단백질을 인식하는 단일클론 항체로, 생식세포 종양 마커 연구에 적합합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 항체이며, 4°C 보관 권장. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PL8-F6 |
| Immunogen | Purified human PLAP protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Placental Alkaline Phosphatase (PLAP) plays an important role in the regulation of inflammatory disease processes. There are four related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney types. PLAP reacts with a membrane-bound isoenzyme (Regan and Nagao type) expressed in the placenta during the third trimester of gestation.
PLAP is a useful marker for identifying testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA). A proposed function of PLAP is matrix mineralization; however, mice lacking functional PLAP show normal skeletal development. PLAP is also linked to hypophosphatasia, a disorder characterized by hypercalcemia and skeletal defects, with severity depending on the specific mutation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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