Thermo Fisher Scientific Alkaline Phosphatase (Placental)/PLAP (Germ Cell Tumor Marker) Monoclonal Antibody (PL8-F6)
인간 PLAP 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. Germ cell tumor marker 연구에 적합하며, 단백질 A/G로 정제된 고순도 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PL8-F6 |
| Immunogen | Purified human PLAP protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Placental Alkaline Phosphatase (PLAP) plays an important role in regulating specific inflammatory disease processes. There are four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney types. PLAP reacts with a membrane-bound isoenzyme (Regan and Nagao type) expressed in the placenta during the third trimester of gestation. It is useful in the identification of testicular germ cell tumors.
Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA). A proposed function of PLAP is matrix mineralization; however, mice lacking this enzyme show normal skeletal development. PLAP has been linked to hypophosphatasia, a disorder characterized by hypercalcemia and skeletal defects. The severity of hypophosphatasia varies depending on the specific mutation involved.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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