Thermo Fisher Scientific Phospho-CaMKII alpha/beta (Thr286) Polyclonal Antibody
Phospho-CaMKII alpha/beta (Thr286) 항체로, 인산화된 CaMKII 단백질을 검출합니다. Western blot 및 IHC에 적합하며, 인간, 마우스, 토끼, 랫트에 반응합니다. 친화 크로마토그래피로 정제된 액상 형태이며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunohistochemistry (IHC) | 1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CAMK2A/B conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2259386 |
Product Specific Information
This antibody is predicted to react with human, mouse, and Xenopus based on 100% sequence homology.
It specifically recognizes the ~50 kDa alpha-CaM Kinase II subunit and the ~60 kDa beta-CaM Kinase II subunit phosphorylated at Thr286 in Western blots of rat brain lysates.
Immunolabeling is blocked by the phosphopeptide antigen but not by the corresponding dephosphopeptide.
Target Information
Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulin-dependent protein kinase that mediates cellular responses to various intercellular signals.
It regulates functions such as synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal organization, and Ca2+ homeostasis.
Phosphorylation of Thr286 generates an autonomously active form of CaM Kinase II, while phosphorylation at Thr305 inhibits its activity and reduces association with the PSD, impacting LTP and learning.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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