Thermo Fisher Scientific SREBP1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 2 µg/mL
• View 1 publication

Gel Shift (GS)

• Tested Dilution: —
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Product Specifications

Product Specific Information

• Predicted to react with porcine based on 100% sequence homology.
• 94% sequence homology with bovine, 88% with zebrafish, and 70% with chicken.
• Suggested positive control: human liver lysate.

Target Information

Sterol regulatory element-binding proteins (SREBPs) are transcription factors belonging to the basic helix-loop-helix leucine zipper family. Three isoforms are identified in mammalian tissues with distinct structures and functions.
SREBP-1a and SREBP-1c (originally cloned as ADD1) are products of alternative promoter usage of the SREBP-1 gene, while SREBP-2 is transcribed from a separate gene.

SREBPs exist as 120 kDa inactive precursors in the endoplasmic reticulum membrane. Upon activation, they are cleaved in the Golgi, producing a mature 60–78 kDa transcriptionally active fragment.
In liver and adipose tissues, SREBPs regulate lipid and cholesterol metabolism by inducing transcription of related genes.
SREBP-1 primarily controls triglyceride synthesis, whereas SREBP-2 is associated with cholesterol synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.