Thermo Fisher Scientific 53BP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Flow Cytometry (Flow)

2 - 5 µg/mL

-

Immunoprecipitation (IP)

Assay-Dependent

-

in situ PLA (PLA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human tumor protein p53-binding protein 1 encoded in exon 11, 12 and 19.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2303638

Product Specific Information

Human samples have been tested in Western Blot and ICC/IF, and mouse samples have been tested in ICC/IF only.

Suggested positive control: U205 cell lysates.

Target Information

53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.