Thermo Fisher Scientific Histone H3 Polyclonal Antibody
다종 생물 반응성을 가진 Rabbit Polyclonal Histone H3 항체로, Western blot, IHC, IP에 사용 가능. 핵 내 히스톤 H3 단백질 검출에 적합하며, 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide within 100 amino acids from N-terminus of human histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: HeLa Nuclear Extract
- Cellular location: Nucleus
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or enable binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes modifications such as methylation, acetylation, and phosphorylation that influence cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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