Thermo Fisher Scientific CXCL10 Polyclonal Antibody, PeproTech
Rat CXCL10(IP-10)을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 적합. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat IP-10 (CXCL10) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930257 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAP
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat IP-10 (CXCL10). The antibody was purified by affinity chromatography using an immobilized Rat IP-10 (CXCL10) matrix.
Sandwich ELISA:
To detect Rat IP-10 (CXCL10) by sandwich ELISA (using 100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When used with PeproTech Biotinylated Anti-Rat IP-10 (CXCL10) (500-P290Bt) as detection antibody, it allows detection of at least 0.2–0.4 ng/well of recombinant Rat IP-10 (CXCL10).
Western Blot:
For Western blot analysis, use 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for recombinant Rat IP-10 is 1.5–3.0 ng/lane under both reducing and non-reducing conditions.
Target Information
IP-10 (interferon-gamma induced protein 10 kD), also known as cytokine responsive gene 2 (Crg-2) or mob-1 in mouse, is a member of the CXC chemokine superfamily. It was first cloned in 1985. Although its biological functions are not fully understood, IP-10 is known to:
- Stimulate monocyte, NK, and T-cell migration
- Regulate T-cell and bone marrow progenitor maturation
- Modulate adhesion molecule expression
- Inhibit angiogenesis
Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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