Thermo Fisher Scientific IL-8 (CXCL8) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-8 (CXCL8). Anti-Human IL-8 (CXCL8)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human IL-8 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-8 (500-P28) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-8.

Western Blot:
To detect Human IL-8 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IL-8 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P28BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor belonging to the CXC chemokine family, binding to IL-8RA and IL-8RB receptors. It functions as a chemoattractant and angiogenic factor, induced by inflammatory stimuli in various cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is stored in Weibel-Palade bodies.
Originally isolated from osteosarcoma cells, IL-8 contains the ELR motif (Glu-Leu-Arg) and signals through CXCR1 and CXCR2 receptors. Other names include NAP-1, GCP-1, MONAP, and protein 3-10C. IL-8 and related CXC chemokines are clustered on chromosome 4q.
IL-8 plays roles in tumor angiogenesis and growth, and in the pathogenesis of bronchiolitis caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.