Thermo Fisher Scientific NCF4 Monoclonal Antibody (OTI12H10), TrueMAB
NCF4 단백질을 타겟으로 하는 Mouse monoclonal antibody로, Human, Mouse, Rat에 반응합니다. IHC(P)에서 1:500 희석으로 사용 가능하며, Lyophilized 형태로 제공됩니다. PBS buffer에 8% trehalose 포함, -20°C 보관 권장.
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Applications
Tested Application and Dilution
- Immunohistochemistry (Paraffin) [IHC (P)]: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI12H10 |
| Immunogen | Full length human recombinant protein of human NCF4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The protein encoded by NCF4 gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase complex, essential for host defense.
It is mainly expressed in myeloid lineage cells and interacts with NCF2 (p67-phox) and NCF1 (p47-phox) to form a complex that activates flavocytochrome b, the catalytic core of the enzyme system.
The PX domain binds phospholipid products of PI(3) kinase, suggesting its role in PI(3) kinase-mediated signaling.
Phosphorylation negatively regulates enzyme activity.
Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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