Thermo Fisher Scientific CLOCK Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

-

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200

View 2 publications 2 publications

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2082586

Product Specific Information

PA1-520 detects CLOCK from human, mouse, rat and hamster tissues.

PA1-520 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from rat brain homogenate. Immunohistochemical staining of CLOCK in hamster brain with PA1-520 results in the staining of the superchiasmatic nucleus.

PA1-520 immunizing peptide corresponds to amino acid residues 11-30 of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-520 immunizing peptide (Cat. # PEP-072) is available for use in neutralization and control experiments.

Target Information

This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.