Thermo Fisher Scientific BMAL1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C D(582) M I D N D Q G S S S P S(594) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BMAL1,uniProtId:Q9WTL8-1,ncbiNodeId:10090,antigenRange:582-594,antigenLength:632,antigenImageFileName:PA1-523_BMAL1_Q9WTL8-1_Rabbit.svg,antigenImageFileNamePDP:PA1-523_BMAL1_Q9WTL8-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2059614

Product Specific Information

PA1-523 detects BMAL1/aryl hydrocarbon nuclear translocator 3 (ARNT3) from hamster and mouse tissues as well as recombinant human BMAL1.

PA1-523 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects a 110 kDa protein which corresponds to the product of a hamster GST-BMAL1 fusion construct overexpressed in E. coli. This antibody detects a non-specific band in U87-MG cell lysates at ~105kDa and in NIH-3T3 cell lysates at ~135kDa.

PA1-523 immunizing peptide corresponds to amino acid residues 582-594 from mouse BMAL1. This sequence is completely conserved between mouse, rat, guinea pig, and human BMAL1. PA1-523 immunizing peptide (Cat. # PEP-075) is available for use in neutralization and control experiments.

Target Information

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.