Thermo Fisher Scientific MIP-1 beta Polyclonal Antibody, Biotin
Human MIP-1 beta에 특이적인 Biotin 결합 폴리클로날 항체로, Western blot 및 ELISA에 적합. Rabbit IgG로 제조되어 높은 특이성과 안정성을 제공. 연구용으로만 사용 가능하며 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific MIP-1 beta Polyclonal Antibody, Biotin
Applications
- Western Blot (WB): 1:2,000–1:10,000
- ELISA (ELISA): 1:20,000–1:100,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human MIP-1 beta Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1beta protein. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only before immediate use.
MIP-1 beta Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis. The antibody was heat-treated at 56°C for 30 minutes.
In ELISA and other immunoreactive assays, this antibody recognizes both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control using similarly diluted normal rabbit IgG is recommended.
Target Information
CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family.
It is involved in chemotactic and proinflammatory effects, and plays a role in homeostasis.
CCL4 is produced by macrophages upon stimulation by bacterial endotoxins and recruits inflammatory cells to sites of inflammation.
Produced by lymphocytes, macrophages, and dendritic cells, CCL4 and CCL3 regulate the trafficking and activation of inflammatory cells.
While both affect monocytes similarly, CCL4 selectively attracts CD4+ lymphocytes and CCL3 attracts CD8+ lymphocytes.
They are potent chemoattractants for B cells, eosinophils, and dendritic cells.
Processed CCL4 can down-modulate CCR5 receptor expression, inhibiting CCR5-mediated HIV-1 entry into T cells.
CCL4 binds with high affinity to CCR5 receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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