Thermo Fisher Scientific VEGF Polyclonal Antibody, Biotin

Thermo Fisher Scientific VEGF Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid; dilute only before immediate use.
• Biotin-conjugated VEGF antibody is purified from monospecific antiserum via delipidation, salt fractionation, ion-exchange chromatography, and dialysis.
• Heated to 56°C for 30 minutes for stabilization.
• Recognizes both native and recombinant human VEGF-165 in cell supernatants and certain body fluids.
• Use a similarly diluted normal rabbit IgG as control.

Target Information

VEGF (Vascular Endothelial Growth Factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis, tumor progression, and metastasis.
It promotes endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability.
The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.
VEGF interacts with tyrosine-kinase receptors FLT1 (VEGFR-1) and FLK1/KDR (VEGFR-2), primarily expressed on endothelial cells, regulating embryonic vasculature development.
An alternatively spliced form, sFLT1, binds VEGF with high affinity and inhibits angiogenesis.
Elevated VEGF levels are associated with POEMS (Crow-Fukase) syndrome, affecting multiple organs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.