Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor 488, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• Custom conjugation available upon request

Product Specific Information

The 236A/E7 antibody reacts with human Foxp3 protein (FORKHEAD BOX P3, SCURFIN, JM2). Foxp3 (49–55 kDa) belongs to the forkhead/winged-helix family of transcriptional regulators and is defective in scurfy mice. It is highly expressed in CD4+CD25+ regulatory T cells (Tregs) and induces a Treg phenotype when ectopically expressed in CD4+CD25− cells.

Intracellular staining and flow cytometric analysis of human PBMCs using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) reveals staining of the CD4+CD25bright population.
The 236A/E7 epitope differs from that of PCH101 (Product #72-5776-40). Recognizes rhesus macaque, sooty mangabey, and cynomolgus macaque.

Applications Reported: Intracellular staining followed by flow cytometric analysis and IHC.
Applications Tested: Pre-titrated and validated using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Recommended usage: 5 µL (0.5 µg)/test (final volume 100 µL; cell number 10⁵–10⁸ cells/test).
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is 381 amino acids long, expressed in CD25+CD4+ regulatory T cells, and absent in CD4−CD8+ T cells. FOXP3 acts as a master regulator and specific marker of Tregs.
Defects in FOXP3 lead to scurfy phenotype in mice and IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans. Transcript variants encoding different isoforms are known. FOXP3 expression in breast and colon cancer cells is regulated by p53 in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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