Thermo Fisher Scientific Myogenin Monoclonal Antibody (F5D), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5-20 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F5D

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1907434

Product Specific Information

Description: This F5D monoclonal antibody reacts with human, mouse, dog, and rat myogenin, a 34-kDa transcription factor. Expressed in skeletal and heart muscle, myogenin is a member of the MyoD family of basic-helix-loop-helix proteins, which also includes MyoD, Myf5, and MRF4. This transcription factor interacts with other helix-loop-helix proteins, which may or may not be muscle-specific. Myogenin plays a significant role in myogenic differentiation, even directing nonmuscle cells to the myogenic lineage. Transforming growth factor-beta (TGFb) and bone morphogenetic protein-2 (BMP2) inhibit myogenin transcriptional activity. Predominantly residing within the nucleus, the subcellular localization of myogenin has been shown to be dependent on differentiation status and cell density. For instance, trafficking of myogenin between the nucleus and cytoplasm has been reported during skeletal muscle differentiation to mediate transcription control.

Applications Reported: This F5D antibody has been reported for use in immunocytochemistry.

Applications Tested: This F5D antibody has been tested by immunocytochemistry of the C2C12 cell line. This can be used at less than or equal to 5-20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.