Thermo Fisher Scientific Syrian Hamster IgG Isotype Control, Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum.

Applications Reported:
Reported for use in flow cytometric analysis and immunohistochemical staining.

Applications Tested:
This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood.
Use the isotype control at the same concentration as the experimental antibody.

Fluorescence Properties:

• Excitation: 488 nm
• Emission: 519 nm
• Laser: Blue Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

The isotype of a primary antibody and the application it is used in can result in background staining.
Primary antibody background noise may be caused by binding to Fc receptors on target cells, non-specific interactions with cellular components, or cell autofluorescence.

Isotype control antibodies serve as negative controls to distinguish non-specific background from specific antibody signals.
They have no relevant specificity to a target antigen and are most commonly used in flow cytometry, but are also applicable in ChIP, IHC, and gel shift assays.

Isotype controls should match the primary antibody species and isotype to ensure accurate determination of specific staining.
When using directly labeled primary antibodies, the isotype control should be conjugated with the same label.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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