Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Ultra Violet 737, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 585, 690
• NovaFluor Red 710, 725, 755
• NovaFluor Yellow 590, 660, 730, 755
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except erythrocytes and platelets. It plays a crucial role in lymphocyte activation via its tyrosine phosphatase domain.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells.
  • Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for UV laser (355 nm) cytometers. Ensure instrument compatibility before use.

Staining Recommendations

• Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) when combining multiple polymer dye-conjugated antibodies to minimize non-specific interactions.
• Protect from light; dye is sensitive to photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (00-8222) or Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
• Compatible with short-term methanol fixation (≤1 hour).

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It regulates Src-family kinases, JAK kinases, and antigen receptor signaling, influencing lymphocyte activation, survival, and apoptosis. CD45 isoforms arise from alternative splicing, differing in extracellular domains and expression patterns across lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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