Thermo Fisher Scientific IL-6 Monoclonal Antibody (MP5-32C11), Biotin, Functional Grade, eBioscience

Applications

ELISA (ELISA)

• Tested Dilution: Assay-dependent
• Publications: View 5 publications

Functional Assay

• Assay-dependent
• Publications: View 2 publications

Product Specifications

Product Specific Information

The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6), a 21–28 kDa cytokine secreted by various cell types.

Applications Reported:
The Functional Grade Biotin anti-mouse IL-6 antibody (clone MP5-32C11) is reported for use as the in vivo capture antibody in the In Vivo Cytokine Capture Assay (IVCCA).
This assay increases the in vivo half-life of cytokines, improving detection sensitivity by 30–1000 fold.

Reference:
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. (2003). The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology, Unit 6.28. John Wiley and Sons, New York.

Applications Tested:
The Functional Grade Biotin MP5-32C11 antibody is endotoxin-free (LAL assay verified) and tested as a detection antibody in sandwich ELISA for mouse IL-6.

• Capture antibody: Purified MP5-20F3 (Cat. 14-7061)
• Standard: Recombinant mouse IL-6 (Cat. 14-8061)
• Recommended detection range: 0.5–2 µg/mL
• Standard curve: 1000 pg/mL – 8.0 pg/mL (doubling dilutions)

Endotoxin:
< 0.05 ng/µg antibody (LAL assay determined)

Storage and Handling:
Use in a sterile environment.

Target Information

IL-6 encodes a cytokine involved in inflammation and B-cell maturation. It acts as an endogenous pyrogen inducing fever in autoimmune or infectious conditions.
Expression occurs at inflammation sites, where IL-6 is secreted into serum and activates transcriptional inflammatory responses via the IL-6 receptor alpha.
IL-6 is implicated in various inflammation-related diseases, including diabetes mellitus susceptibility and systemic juvenile rheumatoid arthritis.
Elevated IL-6 levels are observed in viral infections such as COVID-19 (SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis (Systemic Juvenile).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.