Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Ultra Violet 737, eBioscience
Mouse CD45.2 단백질을 인식하는 단클론 항체로, Brilliant Ultra Violet 737 형광체가 결합되어 Flow Cytometry에 최적화됨. C57BL/6 등 주요 마우스 계통에 적용 가능하며, 높은 형광 안정성과 재현성을 제공. 연구용으로 사용.
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Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), Brilliant Ultra Violet 737, eBioscience™
Applications
- Flow Cytometry (Flow): Tested at ≤ 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Brilliant Ultra Violet™ 737 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | Brilliant Ultra Violet™ 737 |
| Excitation / Emission Max | 350 / 740 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2895968 |
Available Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 805
- Brilliant Violet 421, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 780
- Request custom conjugation
Product Specific Information
The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, DBA, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize by titration.
- Fluorochrome: Brilliant Ultra Violet™ 737 (BUV737), tandem dye emitting at 732 nm; excitation at 355 nm (UV laser required).
- Light Sensitivity: Protect from light to prevent photo-induced oxidation.
- Fixation Compatibility: Samples can be stored up to 3 days in IC Fixation Buffer or 1-step Fix/Lyse Solution at 4°C in dark. Compatible with short-term methanol fixation (<1 hour).
- Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific polymer interactions.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells. It regulates Src-family kinases, lymphocyte development, and antigen signaling. CD45.2 isoforms differ in extracellular domain composition and membrane localization, affecting cell signaling and survival. CD45RA is a related isoform with restricted expression among lymphoid subtypes.
For Research Use Only. Not for use in diagnostic procedures.
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