Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), Brilliant Ultra Violet 737, eBioscience™

Applications

• Flow Cytometry (Flow): Tested at ≤ 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 805
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, DBA, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize by titration.
• Fluorochrome: Brilliant Ultra Violet™ 737 (BUV737), tandem dye emitting at 732 nm; excitation at 355 nm (UV laser required).
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation Compatibility: Samples can be stored up to 3 days in IC Fixation Buffer or 1-step Fix/Lyse Solution at 4°C in dark. Compatible with short-term methanol fixation (<1 hour).
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific polymer interactions.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells. It regulates Src-family kinases, lymphocyte development, and antigen signaling. CD45.2 isoforms differ in extracellular domain composition and membrane localization, affecting cell signaling and survival. CD45RA is a related isoform with restricted expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지