Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet 737, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It plays a key role in lymphocyte activation via tyrosine phosphatase activity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse bone marrow cells (≤0.5 µg per test, 100 µL final volume)
• Recommended: Empirical titration for optimal assay performance

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm, optimized for cytometers with a 355 nm UV laser. Protect from light due to photo-induced oxidation sensitivity.

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific polymer interactions.

Fixation Compatibility:
Samples can be fixed in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark. Compatible with short-term methanol-based fixation (<1 hour).

Laser Information:

• Excitation: 355 nm
• Emission: 732 nm
• Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, Leukocyte Common Antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It regulates Src-family kinases and antigen receptor signaling in T and B cells, playing roles in cell survival and immune regulation. Multiple isoforms exist due to alternative splicing, differing in extracellular domains while sharing transmembrane and cytoplasmic regions.

For Research Use Only. Not for use in diagnostic procedures.

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