Thermo Fisher Scientific Calbindin D28K Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

5 µg/mL

-

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

28 kDa calbindin-D protein purified from rat kidney. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calbindin D28K,uniProtId:P07171-1,ncbiNodeId:10114,antigenRange:1-261,antigenLength:261,antigenImageFileName:PA1-931_Calbindin_D28K_P07171-1_Rabbit.svg,antigenImageFileNamePDP:PA1-931_Calbindin_D28K_P07171-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2068509

Product Specific Information

PA1-931 detects calbindin D-28k protein in rat samples.

PA1-931 has successfully been used in Western blot , ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 28 kDa protein representing calbindin D from rat cerebellum and rat kidney extract. Immunohistochemical staining on paraffin sections demonstrates that calbindin D is localized to the cytoplasm in epithelial cells of distal tubules in human kidney cortex after staining with PA1-931.

The PA1-931 immunizing protein corresponds to the purified 28 kDa calbindin-D protein from rat kidney.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

Target Information

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.