Thermo Fisher Scientific S100A1 Polyclonal Antibody
S100A1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용 가능. Human, Mouse, Rat 등 반응. 항원 친화 크로마토그래피로 정제되었으며, -20°C 보관. 진단용이 아닌 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | 2 publications |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL | 2 publications |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | 1 publication |
| ELISA | Assay-dependent | 1 publication |
| Immunoprecipitation (IP) | 5 µg/mL | - |
| Dot Blot (DB) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified S100-alpha protein from human pectoral muscle cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 20 mg/mL BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2183210 |
Product Specific Information
PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples, and shows no cross-reactivity with S100-beta protein.
It has been validated for use in Western blot, ELISA, immunofluorescence, immunocytochemistry, immunoprecipitation, and immunohistochemical procedures.
By Western blot, it detects a 10 kDa protein representing S100-alpha from human muscle.
Immunohistochemistry on paraffin sections shows S100-alpha localization in serous cells, myoepithelial cells, and epithelial cells of intercalated ducts in the parotid gland.
S100 protein is relatively small; electrophoresis using tricine-SDS-PAGE gels and transfer to a nylon membrane is recommended.
The immunizing protein corresponds to purified S100-alpha from human pectoral muscle cells.
Reconstitute in 100 µL PBS to obtain a 1 mg/mL stock solution.
Target Information
S100 belongs to the calcium-binding protein family, including calmodulin and troponin C.
S100A consists of alpha and beta chains, while S100B consists of two beta chains.
S100 proteins are also expressed in antigen-presenting cells such as Langerhans cells in skin and interdigitating reticulum cells in lymph nodes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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