Thermo Fisher Scientific S100A1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

5 µg/mL

-

Dot blot (DB)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified S100-alpha protein from human pectoral muscle cells. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:S100A1,uniProtId:P23297-1,ncbiNodeId:9606,antigenRange:1-94,antigenLength:94,antigenImageFileName:PA1-932_S100A1_P23297-1_Rabbit.svg,antigenImageFileNamePDP:PA1-932_S100A1_P23297-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 20mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2183210

Product Specific Information

PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein.

PA1-932 has successfully been used in Western blot, ELISA, immunofluorescence, immunocytochemistry, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932.

S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.

The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

Target Information

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.