Thermo Fisher Scientific PDH Antibody Cocktail

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunocytochemistry (ICC/IF)

1:250

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / Ig

Class

Cocktail

Type

Antibody

Clone

Cocktail

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline, pH 7.5

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2533826

Product Specific Information

Anti-PDH Western Blot Kit; Western Blot kit (Cocktail of monoclonal antibodies) specific to Human, bovine PDH. This antibody is validated for use in Western Blot.

C-V-OSCP (clone ) 4C11C10D12 - Antigen Human rec. mature OSCP
Amount: 12.5 µg , Working concentration: 0.5 µg/mL

PDH-E1-alpha (clone) 9H9AF5 - Human rec. PDH-E1-a
Amount: 25 µg , Working concentration: 1 µg/mL

PDH-E1-beta (clone) 17A5E2H8 - Porcine PDH
Amount: 62.5 µg, Working concentration: 2.5 µg/mL

PDH-E2 (clone) 15D3G9C11 - Porcine PDH
Amount: 25 µg, Working concentration: 1 µg/mL

PDH-E2/E3bp (clone) 13G2AE2BH5 - Porcine PDH
Amount: 25 µg, Working concentration: 1 µg/mL

Target Information

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.