Thermo Fisher Scientific PDHA1 Monoclonal Antibody (8D10E6)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

-

Flow Cytometry (Flow)

1 µg/mL

-

Functional Assay (Functional)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Fruit fly, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

8D10E6

Immunogen

Human recombinant PDH-E1-alpha

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2533825

Product Specific Information

When performing ICC and IF heat-induced antigen-retrieval improves signal.

When performing Flow Cytometry Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Positive Control: WB: Isolated mitochondria from human, bovine, rat and mouse heart. HepG2 (human liver hepatocellular carcinoma cell line) cell lysate. FACS: HeLa (human epithelial cell line from cervix adenocarcinoma) and HL-60 (human promyelocytic leukemia cell line) cells.

Target Information

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.