Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), Functional Grade, eBioscience

Applications and Publications

Immunohistochemistry (IHC)

• Tested Dilution: –
• View 3 publications

Flow Cytometry (Flow)

• Tested Dilution: –
• View 64 publications

Neutralization (Neu)

• Tested Dilution: Assay-Dependent
• View 1 publication

Functional Assay (Functional)

• Tested Dilution: Assay-Dependent
• View 1 publication

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4⁺ T cells. TNF-α exhibits cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported:
Reported for use in ELISA, neutralization, and immunohistochemistry.

Applications Tested:
The Functional Grade Purified MP6-XT22 antibody has been tested by LAL assay for low endotoxin levels and for neutralization of TNF-α bioactivity.
At 1.25 µg/mL, it neutralizes 50% of the biological effects of 1.0 ng/mL mouse TNF-α in an assay of TNF-α-induced cytotoxicity of L929 cells.
Detailed protocols are available in the BestProtocols® Cytokine Bioassays Guide.

Recommendations:
Careful titration is recommended for optimal performance in the assay of interest.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10%, determined by HPLC.

Target Information

TNF-α is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF-α regulates immune cell activity, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It forms multimers (dimers, trimers, or pentamers) and appears as a 17 kDa band under non-reducing SDS-PAGE conditions.
Knockout studies in mice indicate TNF-α’s neuroprotective functions and its role in tumor necrosis. It is involved in immune responses to bacterial, viral, parasitic, and fungal infections, and implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.