Thermo Fisher Scientific IFN gamma Monoclonal Antibody (MD-1), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

1-4 µg/mL

-

Neutralization (Neu)

-

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MD-1

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866278

Product Specific Information

Description: The MD-1 antibody reacts with human, rhesus, cynomolgus and baboon interferon-gamma (IFN gamma), the signature Th1 cytokine, which is produced mainly by activated T cells, NK cells and B cells. IFN gamma is a dimeric protein. IFN gamma has antiviral and antiparasitic activities and also inhibits the proliferation of normal and transformed cells.

Applications Reported: The MD-1 antibody has been reported for use in intracellular staining and flow cytometric analysis, cytokine neutralization, immunohistochemical staining, ELISA Capture, and ELISPOT Capture. Fluorochrome conjugated 4S.B3 antibody is recommended for use in intracellular staining and flow cytometry.

Applications Tested: The MD-1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interferon gamma (IFN gamma) in combination with the biotinylated 4S.B3 (13-7319) antibody for detection and recombinant human IFN gamma (39-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

The FG Purified format of the MD-1 antibody has been tested as the capture antibody in an ELISPOT assay (in conjunction with the biotinylated 4S.B3 antibody) and for neutralization of IFN gamma biological activity. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/mL.

For in vitro neutralization, the MD-1 antibody at 30 µg/mL has been found to neutralize by 50% the effect of 1 ng/mL human IFN gamma. A more potent neutralizing antibody is clone NIB42.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.