Thermo Fisher Scientific IFN gamma Monoclonal Antibody (MD-1), Functional Grade, eBioscience
인간 IFN-γ를 인식하는 MD-1 단클론 항체로, ELISA, ELISPOT, 중화 및 세포내 염색에 적합합니다. 1 mg/mL 액상 형태이며 4°C에서 보관합니다. 고순도(>90%) 및 낮은 엔도톡신 수준(<0.001 ng/µg)으로 연구용에 적합합니다.
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Monoclonal Antibody (MD-1), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | - | 2 |
| ELISA (ELISA) | 1–4 µg/mL | - |
| Neutralization (Neu) | - | 2 |
| Functional Assay (Functional) | Assay-dependent | 1 |
| In vitro Assay (IV) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MD-1 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2866278 |
Additional Formats
Product Specific Information
The MD-1 antibody reacts with human, rhesus, cynomolgus, and baboon interferon-gamma (IFN-γ), a signature Th1 cytokine produced mainly by activated T cells, NK cells, and B cells. IFN-γ is a dimeric protein with antiviral and antiparasitic activities and inhibits proliferation of normal and transformed cells.
Applications Reported:
Intracellular staining, flow cytometry, cytokine neutralization, immunohistochemistry, ELISA capture, and ELISPOT capture.
Fluorochrome-conjugated 4S.B3 antibody is recommended for intracellular staining and flow cytometry.
Applications Tested:
- ELISA Capture: Tested as a capture antibody for human IFN-γ, used with biotinylated 4S.B3 (13-7319) for detection and recombinant IFN-γ (39-8319) as standard. Recommended range: 1–4 µg/mL.
Include a standard curve (1000 pg/mL – 8 pg/mL) for each plate. - ELISPOT Capture: Tested with biotinylated 4S.B3 antibody; suitable range 1–4 µg/mL.
- Neutralization: At 30 µg/mL, neutralizes 50% of the effect of 1 ng/mL human IFN-γ. Clone NIB42 is more potent.
Storage and Handling:
Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg (LAL assay).
Aggregation: <10% (HPLC).
Target Information
IFN-γ (Interferon gamma, Type II interferon)
A macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens and cytokines.
- Structure: Dimeric glycoprotein (~45 kDa), composed of two 146 amino acid subunits.
- Functions: Antiviral and antiproliferative activity, induction of MHC I/II, macrophage activation, and enhanced immunoglobulin secretion.
- Species specificity: Human IFN-γ does not cross-react with mouse IFN-γ.
- Regulation: Upregulated by IL-2, FGF basic, EGF; downregulated by vitamin D3 or DMN.
- Associated with cytokine regulation and JAK-STAT pathway activation.
- Mutations linked to aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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