Thermo Fisher Scientific HO-1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400

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Immunohistochemistry (IHC)

1:1,000

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Immunoprecipitation (IP)

1:100

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735913

Product Specific Information

5 µg/mL of PA5-77834 was sufficient for detection of HO-1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |Detects approximately 33kDa.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.