Thermo Fisher Scientific CPI17 alpha Polyclonal Antibody
CPI17 alpha 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, ELISA 등에 사용 가능. Human 및 Mouse 시료에 반응하며, Protein A로 정제된 액상 제품. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human CPI17 alpha, amino acids 5–100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphatase. It acts as a PKC-potentiated inhibitory protein of protein phosphatase-1, predominantly expressed in smooth muscle. Phosphorylation at Threonine 38 by PKC or Rho-kinase enhances its inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and may be involved in calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. Dual phosphorylation occurs at Serine 12 and Threonine 38 by MYPT-associated kinase (M110 kinase).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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