Thermo Fisher Scientific FEN1 Monoclonal Antibody (4E7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Chicken

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E7

Immunogen

Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FEN1,uniProtId:P39748-1,ncbiNodeId:9606,antigenRange:1-380,antigenLength:380,antigenImageFileName:MA1-23228_FEN1_P39748-1_House_mouse.svg,antigenImageFileNamePDP:MA1-23228_FEN1_P39748-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.73 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_558904

Product Specific Information

Recommended positive controls: MCF7.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5` flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.