Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), FITC, eBioscience
Mouse CD62L (L-Selectin) 단백질을 인식하는 MEL-14 클론의 FITC 결합 단일클론 항체입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.5 µg/test 농도로 사용됩니다. 형광 특성은 488 nm 여기, 520 nm 방출이며, 4°C 암저장 조건을 권장합니다.
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), FITC, eBioscience
Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 108 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEL-14 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465108 |
Available Formats
- Alexa Fluor 561
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 615, 737, 805
- Brilliant Violet 421, 480, 605, 650, 786
- eFluor 450
- Functional Grade
- NovaFluor Blue 510, 555, 585, 610-30S, 660-40S
- NovaFluor Red 710
- NovaFluor Yellow 590, 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 702, 780
- Custom conjugation available upon request
Product Specific Information
The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, sulfated sialylated glycoproteins such as CD34, glycam-1, and MAdCam-1, mediating lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume).
Recommended careful titration for optimal performance. - Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor. It includes:
- Aminoterminal C-type lectin binding domain
- EGF-like domain
- Two short consensus repeat sequences (SCR)
- Spacer, transmembrane, and cytoplasmic regions
CD62L is constitutively expressed on most leukocytes including lymphocytes (except many memory T cells), monocytes, and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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