Thermo Fisher Scientific CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), FITC, eBioscience
CD68 단백질을 인식하는 FITC 결합 단클론 항체로, 인간 유래 세포의 유세포분석 및 면역염색에 적합합니다. 488 nm에서 여기되고 520 nm에서 방출되며, 친화크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다. 연구용 시약으로만 사용됩니다.
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Thermo Fisher Scientific CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), FITC, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 publications |
| Immunocytochemistry (ICC/IF) | 0.125 µg/mL | 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | 19 publications |
| Immunomicroscopy (IM) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), FITC |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioY1/82A (Y1/82A) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151698 |
Available Formats
- Unconjugated
- Alexa Fluor 647
- Biotin
- PE
- PE-Cyanine7
- PE-eFluor 610
- Request custom conjugation
Product Specific Information
The eBioY1/82A antibody reacts with CD68, a sialomucin family protein closely related to lysosomal-associated membrane proteins (LAMPs) and scavenger receptors. CD68 is mainly intracellular, localized in late endosomal compartments, but also found on the surface of myeloid-derived cells such as monocytes, macrophages, dendritic cells, and subsets of CD34+ progenitor cells.
Staining with eBioY1/82A shows surface staining on B cells and a minor fraction of monocytes, while intracellular staining detects CD68 in about 50% of B cells and most monocytes.
Applications Reported: Flow cytometric analysis
Applications Tested: Intracellular staining and flow cytometry of normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test in 100 µL final volume.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein mainly expressed in lysosomes of monocytes and macrophages, with lower expression in dendritic cells and granulocytes. It plays roles in phagocytic activity, lysosomal metabolism, and cell-pathogen interactions. CD68 expression is also noted in interdigitating reticulum cells, histiocytic lymphomas, AML, and granulocytic sarcoma. Elevated CD68 levels have been observed in CD34+ cells in several human malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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