Thermo Fisher Scientific Nanog Monoclonal Antibody (eBioMLC-51), eFluor 660, eBioscience
마우스 Nanog 단백질을 특이적으로 인식하는 단클론 항체로, 줄기세포의 미분화 상태 분석에 적합합니다. eFluor 660 형광 표지로 유세포 분석, 면역형광, Western blot 등에 사용 가능합니다. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific Nanog Monoclonal Antibody (eBioMLC-51), eFluor 660, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 8 publications |
| Immunohistochemistry (IHC) | Assay-Dependent | 7 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | 29 publications |
| Flow Cytometry (Flow) | 0.06 µg/test | 8 publications |
| ChIP assay (ChIP) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMLC-51 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651/668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574244 |
Product Specific Information
The eBioMLC-51 monoclonal antibody recognizes mouse Nanog, a homeobox transcription factor essential for maintaining pluripotency in embryonic stem cells. Nanog expression is specific to early embryos, the inner cell mass of the blastocyst, ES cells, and EG cells, and is downregulated upon differentiation.
Nanog acts as a transcriptional activator with two activation domains (CD2, WR) in the C-terminus and one in the N-terminus. Immunoblotting with eBioMLC-51 shows a ~45 kDa band in F9 cell lysates but not in NIH3T3 or spleen lysates. For intracellular staining, use the eBioscience Foxp3 Staining Buffer Set (Product #00-5523).
Applications Reported: Intracellular staining with flow cytometry, western blotting, microscopy, immunohistochemistry, and immunocytochemistry.
Applications Tested:
- ICC: ≤10 µg/mL
- Flow cytometry: ≤0.06 µg/test using Foxp3/Transcription Factor Staining Buffer Set.
A test is defined as the antibody amount used in 100 µL final volume, typically 10⁵–10⁸ cells/test. Optimize antibody concentration for best performance.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
NANOG (Nanog homeobox) is a transcription factor regulating pluripotency and differentiation in embryonic stem cells. NANOG mRNA is expressed in pluripotent cell lines and absent in differentiated cells. Human NANOG shares 52% overall identity with mouse NANOG and 85% in the homeodomain.
Human NANOG maps to 12p13.31; mouse NANOG to 6F2. NANOG expression occurs in the inner cell mass of the blastocyst. It promotes self-renewal and proliferation of ES cells and is implicated in iPS cell generation with Sox2, Klf4, and c-Myc expression. NANOG dysfunction is linked to teratocarcinoma and germ cell cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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