Thermo Fisher Scientific HDAC1 Polyclonal Antibody
HDAC1 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, ChIP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태. 인간 및 생쥐 반응성. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human HDAC-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549912 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with the primary antibody for 10 minutes at room temperature.
Suggested positive controls: tonsil or breast carcinoma tissue.
Target Information
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, are key in regulating eukaryotic gene expression.
HDAC1 is part of the histone deacetylase complex and interacts with retinoblastoma tumor-suppressor protein, playing a crucial role in cell proliferation and differentiation.
Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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