Thermo Fisher Scientific Cytokeratin 2e Polyclonal Antibody
Rabbit polyclonal antibody targeting Cytokeratin 2e, validated for WB, IHC, and ICC applications. High specificity to human protein with predicted cross-reactivity to dog and chimpanzee. Supplied as a liquid, unconjugated form with antigen affinity pur...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 143–529 of Cytokeratin 2 (Uniprot ID: P35908) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.67 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152031 |
Product Specific Information
- Recommended positive control: A431
- Predicted reactivity: Dog (82%), Chimpanzee (99%)
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
Cytokeratins are a subfamily of intermediate filament proteins characterized by significant biochemical diversity. In human epithelial tissues, at least 20 different cytokeratin polypeptides are expressed, ranging in molecular weight from 40 kDa to 68 kDa and isoelectric pH from 4.9 to 7.8. The expression pattern of cytokeratins varies depending on the type and differentiation state of the epithelium.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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