Thermo Fisher Scientific MCP-3 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine MCP-3 (CCL7) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL7 (MCP3),uniProtId:Q03366-1,ncbiNodeId:10090,antigenRange:24-97,antigenLength:97,antigenImageFileName:500-P116GBT-500UG_CCL7_MCP3_Q03366-1_Goat.svg,antigenImageFileNamePDP:500-P116GBT-500UG_CCL7_MCP3_Q03366-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine MCP-3 (CCL7). Anti-Murine MCP-3 (CCL7)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Murine MCP-3 (CCL7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-3 (CCL7) (500-P116G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MCP-3 (CCL7).

Western Blot: To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P116GBT-1MG will be provided as 2 x 500 µg

Target Information

This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.